A 40-mhz Frequency Multiplexed Electronic System for Multicolor Droplet Flow Cytometry
نویسندگان
چکیده
Multiparameter flow cytometry is commonly used for the analysis of cells and droplet microreactors. However, their cost and complexity limits its applications in point-of-care systems. This paper demonstrates a high speed frequency multiplexed electronic detector for multicolor flow cytometry. The system can detect multiple fluorophores on a single PMT, rather than one per fluorophore. The detector is operating at up to 40MHz, enabling a multi-channel bandwidth of >200 KHz as required by cell cytometry. The system successfully distinguishes alternating water-in-oil droplets containing fluorescein or Alex 680 alternately at throughputs of 300 drops/sec and single fluorescein droplets at 2800 drop/sec.
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